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마우스 기본 Hypothalamic 뉴런 Deconvolution 현미경을 사용 하 여 매매 GLUT4 단백질의 라이브 이미지

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

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마우스 기본 Hypothalamic 뉴런 Deconvolution 현미경을 사용 하 여 매매 GLUT4 단백질의 라이브 이미지

9,213 Views

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08:47 min

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December 07, 2017

DOI:

10.3791/56409-v

DOI:

10.3791/56409-v

•

08:47 min

December 07, 2017

•

3 Views

Chun Austin Changou^1,2,3, Reni Ajoy^4,5, Szu-Yi Chou^4,5,6

¹The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology,Taipei Medical University, ²Integrated Laboratory, Center of Translational Medicine,Taipei Medical University, ³Core Facility,Taipei Medical University, ⁴The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, ⁵Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, ⁶TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology,Taipei Medical University

Summary

이 프로토콜 설명 관찰 실시간 녹색 형광 단백질 (GFP)의 태그 인슐린 자극 및 인슐린-GLUT4에 CCR5의 생물학 역할의 특성에 따라 매매 하는 포도 당 운송업 자 4 (GLUT4) 단백질에 대 한 Deconvolution 현미경으로 통로 신호.

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