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Immagini in diretta della proteina GLUT4 tratta di neuroni ipotalamici primario del Mouse usando la microscopia di Deconvolution
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December 07, 2017
DOI:
1The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology,Taipei Medical University, 2Integrated Laboratory, Center of Translational Medicine,Taipei Medical University, 3Core Facility,Taipei Medical University, 4The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 5Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 6TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology,Taipei Medical University
Summary
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Questo protocollo descrive una tecnica per osservazione di in tempo reale verde fluorescenza Protein (GFP) contrassegnati 4 di trasportatore del glucosio (GLUT4) proteina traffico su stimolazione dell'insulina e la caratterizzazione del ruolo biologico di CCR5 in insulina – GLUT4 via con microscopia di Deconvolution di segnalazione.
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Changou, C. A., Ajoy, R., Chou, S. Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy. J. Vis. Exp. (130), e56409, doi:10.3791/56409 (2017).
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