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血脑屏障的高分辨率共聚焦成像: 成像、3D 重建和 Transcytosis 的量化
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis
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血脑屏障的高分辨率共聚焦成像: 成像、3D 重建和 Transcytosis 的量化

11,148 Views

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10:30 min

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November 16, 2017

DOI:

10.3791/56407-v

DOI:
10.3791/56407-v

•

10:30 min
November 16, 2017

•

2 Views
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1Roche Pharma Research and Early Development (pRED), Neuroimmunology,Roche Innovation Center Basel

Tags

Blood-brain BarrierConfocal Microscopy3D ReconstructionTranscytosisImmunofluorescenceFree-floating SectionsEndothelial CellsNeurovascular UnitSubcellular OrganellesQuantificationImage Analysis

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Cite this Article

Villaseñor, R., Collin, L. High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis. J. Vis. Exp. (129), e56407, doi:10.3791/56407 (2017).

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