This article describes a protocol for the large-scale axenic isolation and collection of free-swimming miracidia of the human blood fluke Schistosoma mansoni and their subsequent processing for introduction into in vitro culture.
Dinguirard, N., Heinemann, C., Yoshino, T. P. Mass Isolation and In Vitro Cultivation of Intramolluscan Stages of the Human Blood Fluke Schistosoma Mansoni. J. Vis. Exp. (131), e56345, doi:10.3791/56345 (2018).