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A Simple Neuronal Mechanical Injury Methodology to Study Drosophila Motor Neuron Degeneration
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Neuroscience
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JoVE Journal Neuroscience
A Simple Neuronal Mechanical Injury Methodology to Study Drosophila Motor Neuron Degeneration

A Simple Neuronal Mechanical Injury Methodology to Study Drosophila Motor Neuron Degeneration

DOI:
10.3791/56128-v

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04:18 min

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July 19, 2017

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1Department of Biological Sciences,Quinnipiac University

Chapters

  • 00:05Title
  • 00:41Drosophila Larvae Selection and Preparation
  • 01:30Mechanical Injury and Larvae Treatment
  • 03:16Results: Mechanical Neuronal Injury Enables Temporal Dissection of Neurodegenerative Events
  • 03:53Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a simple and widely accessible method to injure segmental nerves in Drosophila larvae to visualize and quantify neurodegeneration of motor neurons at the neuromuscular junction (NMJ) of third instar larvae.

Tags

DrosophilaMotor NeuronNeurodegenerationMechanical InjuryLarvaSegmental NervesCuticleForcepsAnesthetizingDissecting MicroscopeFruit Juice AgarYeast Paste

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