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C. Elegans Darm als ein Modell für die interzelluläre Lumen Morphogenese und In Vivo polarisiert Membrane Biogenesis Ebene der einzelligen: Kennzeichnung durch Antikörper Färbung, RNAi Verlustfunktion Analyse und Bildgebung
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October 03, 2017
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C. ElegansIntestineIntercellular LumenMembrane BiogenesisPolarized MembraneSingle-cell LevelAntibody StainingRNAiLoss-of-function AnalysisImagingMorphogenesisApical MembraneBasolateral MembranePolarityTubulogenesisExcretory CanalTransgenic AnimalsFluorescent Fusion ProteinsLive ImagingImmunohistochemistryMembrane MarkersPromoters
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Zhang, N., Khan, L. A., Membreno, E., Jafari, G., Yan, S., Zhang, H., Gobel, V. The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging. J. Vis. Exp. (128), e56100, doi:10.3791/56100 (2017).
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