Here, we present a step-wise protocol for the dispersion of nanomaterials in aqueous media with real-time characterization to identify the optimal sonication conditions, intensity, and duration for improved stability and uniformity of nanoparticle dispersions without impacting the sample integrity.
Kaur, I., Ellis, L., Romer, I., Tantra, R., Carriere, M., Allard, S., Mayne-L'Hermite, M., Minelli, C., Unger, W., Potthoff, A., Rades, S., Valsami-Jones, E. Dispersion of Nanomaterials in Aqueous Media: Towards Protocol Optimization. J. Vis. Exp. (130), e56074, doi:10.3791/56074 (2017).