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水平凝胶电泳用于增强蛋白质 - RNA复合物的检测
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Biochemistry
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JoVE Journal Biochemistry
Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
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水平凝胶电泳用于增强蛋白质 - RNA复合物的检测

11,650 Views

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06:36 min

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July 28, 2017

DOI:

10.3791/56031-v

DOI:
10.3791/56031-v

•

06:36 min
July 28, 2017

•

4 Views
, , , ,
1Department of Biomolecular Chemistry,University of Wisconsin School of Medicine and Public Health, 2Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School

Tags

Horizontal Gel ElectrophoresisProtein-RNA ComplexesRNA BiochemistryRNA-protein InteractionsNative Gel ElectrophoresisGel BoxTBE Running BufferAcrylamide GelBinding ReactionFluorescently Labeled RNABicaudal-c Protein

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Dowdle, M. E., Imboden, S. B., Park, S., Ryder, S. P., Sheets, M. D. Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes. J. Vis. Exp. (125), e56031, doi:10.3791/56031 (2017).

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