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Developmental Biology
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臍帯血由来誘導多能性幹細胞からの軟骨形成ペレット形成
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JoVE Journal
Developmental Biology
Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
臍帯血由来誘導多能性幹細胞からの軟骨形成ペレット形成
DOI:
10.3791/55988-v
•
12:10 min
•
June 19, 2017
•
Yoojun Nam
,
Yeri Alice Rim
,
Ji Hyeon Ju
1
CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary’s Hospital, College of Medicine
,
The Catholic University of Korea
,
2
Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary’s Hospital, Institute of Medical Science, College of Medicine
,
The Catholic University of Korea
Chapters
00:05
Title
01:08
iPSC Generation from Cord Blood
02:31
EB Generation
04:43
Outgrowth Cell Induction from EBs
05:45
Chondrogenic Pellet Formation
07:19
Chondrogenic Pellet Characterization by Staining
10:01
Results: Relative Anaysis Results of Chondrogenic Pellets Generated from CBMC-iPSCs
11:33
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、臍帯血単核球由来ヒト由来多能性幹細胞からの軟骨分化のプロトコールを提案する。
Tags
Chondrogenic Pellet
Cord Blood-derived
Induced Pluripotent Stem Cells
Cartilage Repair
IPSCs
Cell Culture
Cell Counting
Centrifugation
Sendai Virus
IPSC Medium
E8 Medium
EDTA
Cell Harvesting
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