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Lokalisatie van Receptorgenen van Reukstof in Locustantenne door RNA<em> In situ</em> Hybridisatie

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

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Lokalisatie van Receptorgenen van Reukstof in Locustantenne door RNA<em> In situ</em> Hybridisatie

7,953 Views

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09:30 min

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July 13, 2017

DOI:

10.3791/55924-v

DOI:

10.3791/55924-v

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09:30 min

July 13, 2017

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2 Views

Xiao Xu, Yinwei You², Long Zhang

¹Department of Entomology,China Agricultural University, ²Bio-tech Research Center,Shandong Academy of Agricultural Sciences

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Odorant Receptor Genes RNA In Situ Hybridization Locust Antennae Digoxigenin Labeled Probes Biotin Labeled Probes Freezing Microtome Cryostat Sections 4% PFA 0.2 M HCl 1X PBS 1% Triton X-100 Formamide Antisense Probe Sense Probe 0.1X SSC 1X TBS 1% Blocking Reagent Chromogenic Detection

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