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New Methods to Study Gustatory Coding
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
New Methods to Study Gustatory Coding

New Methods to Study Gustatory Coding

DOI:
10.3791/55868-v

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10:59 min

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June 29, 2017

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, , ,
1National Institute of Child Health and Human Development (NICHD),National Institutes of Health (NIH), 2Max Planck Institute for Brain Research

Chapters

  • 00:05Title
  • 01:03Preparing M. sexta for Brain Surgery
  • 04:22Revealing the Subesophageal Zone (SEZ) and Maxillary Nerves
  • 06:00Tastant Delivery System and Extracellular Tetrode Recording
  • 08:45Results: Tetrode Recordings of Stimulated Gustatory Receptor Neurons
  • 10:03Conclusion

Summary

Automatic Translation

Automatic Translation

We present three new methods to study gustatory coding. Using a simple animal, the moth Manduca sexta (Manduca), we describe a dissection protocol, the use of extracellular tetrodes to record the activity of multiple gustatory receptor neurons, and a system for delivering and monitoring precisely timed pulses of tastants.

Tags

AI-powered Writing AssistantGustatory CodingIn Vivo RecordingsGustatory Receptor NeuronsTaste StimuliDissection ProcedureInsect PreparationProboscis ManipulationBrain StabilizationSaline Perfusion

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