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Developing HiPSC Derived Serum Free Embryoid Bodies for the Interrogation of 3-D Stem Cell Cultures Using Physiologically Relevant Assays
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Neuroscience
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JoVE Journal Neuroscience
Developing HiPSC Derived Serum Free Embryoid Bodies for the Interrogation of 3-D Stem Cell Cultures Using Physiologically Relevant Assays

Developing HiPSC Derived Serum Free Embryoid Bodies for the Interrogation of 3-D Stem Cell Cultures Using Physiologically Relevant Assays

DOI:
10.3791/55799-v

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10:43 min

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July 20, 2017

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, ,
1The Hussman Institute for Autism

Chapters

  • 00:05Title
  • 01:04Generation of Neural Progenitor Cell
  • 04:02Serum-free Embryoid Body (SFEB) Induction
  • 06:01Recording Neuronal Activity in SFEBs using MEAs
  • 08:09Results: SFEB Characteristics
  • 09:51Conclusion

Summary

Automatic Translation

Automatic Translation

Here we report a protocol to develop a three-dimensional (3-D) system from human induced-pluripotent stem cells (hiPSCs) called the serum free embryoid body (SFEB). This 3-D model can be used like an organotypic slice culture to model human cortical development and for the physiological interrogation of developing neural circuits.

Tags

3D Cell CultureHiPSCEmbryoid BodiesSerum-freeCortical DevelopmentNeurological DisordersDrug DiscoveryNeurodevelopmentNeuroimmune FunctionNeural InformationFetal CellsStem Cell DifferentiationNeuro Precursor Cells

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