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Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
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JoVE Journal Chemistry
Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

DOI:
10.3791/55778-v

•

08:41 min

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July 14, 2017

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, , ,
1Pharmasan Labs, Inc., 2NeuroScience, Inc.

Chapters

  • 00:05Title
  • 01:41Solid Phase Extraction Procedure
  • 05:00LC-MS/MS Analysis
  • 06:17Results: LC-MS/MS Determination of 3-Nitrotyrosine in Human Urine
  • 07:47Conclusion

Summary

Automatic Translation

Automatic Translation

A selective and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method coupled with an efficient solid phase extraction on a mixed-mode cation-exchange (MCX) 96-well microplate was developed for the measurement of free 3-nitrotyrosine (3-NT) in human urine with high throughput, which is suitable for clinical applications.

Tags

3-nitrotyrosineUrineSolid Phase ExtractionLC-MS/MSOxidative StressBiomarkerClinical Application

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