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将斑马鱼小儿脑肿瘤移植入免疫能力的宿主，用于长期研究肿瘤细胞行为和药物反应
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Transplantation of Zebrafish Pediatric Brain Tumors into Immune-competent Hosts for Long-term Study of Tumor Cell Behavior and Drug Response
Please note that all translations are automatically generated. Click here for the English version.

将斑马鱼小儿脑肿瘤移植入免疫能力的宿主，用于长期研究肿瘤细胞行为和药物反应

DOI:
10.3791/55712-v

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09:43 min

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May 17, 2017

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, , , , , , ,
1Department of Oncological Sciences and Huntsman Cancer Institute,University of Utah School of Medicine, 2Department of Dermatology,University of Utah Health Sciences Center, Salt Lake City

Chapters

  • 00:05Title
  • 01:07Preparing Equipment for Microinjection
  • 02:46Making a Tumor Cell Suspension
  • 04:22Injecting Tumor Suspension into the Fourth Ventricle of a 2-dpf Embryo
  • 07:25Results: Brain Tumor Transplantation in Zebrafish
  • 08:57Conclusion

Summary

Automatic Translation

Automatic Translation

癌细胞的移植是识别癌症机制和治疗反应的重要工具。目前的技术取决于免疫力不足的动物。在这里，我们描述了一种将斑马鱼肿瘤细胞移植到免疫感受态胚胎中的方法，用于长期分析肿瘤细胞行为和体内药物反应。

Tags

ZebrafishPediatric Brain TumorsImmune-competent HostsTumor Cell BehaviorDrug ResponseInvasionDisseminationBrain Cancer BiologyTherapyTumor BurdenAgaroseMethylene BlueInjection PlateNeedle PullerCapillariesRazor BladeTumor MassCell SuspensionCentrifugation

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