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Isolation of the Side Population in Myc-induced T-cell Acute Lymphoblastic Leukemia in Zebrafish
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of the Side Population in Myc-induced T-cell Acute Lymphoblastic Leukemia in Zebrafish

Isolation of the Side Population in Myc-induced T-cell Acute Lymphoblastic Leukemia in Zebrafish

English

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6,471 Views

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09:06 min

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May 04, 2017

DOI:

10.3791/55711-v

DOI:
10.3791/55711-v

•

09:06 min
May 04, 2017

•

1 Views
, , ,
1Department of Pediatrics, Section of Hematology-Oncology,The University of Chicago, 2Department of Biology and Biotechnology,Worcester Polytechnic Institute

Summary

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Here, we describe a technique to isolate the side population cells from a zebrafish model of myc-induced T-cell acute lymphoblastic leukemia (T-ALL). This side population assay is highly sensitive and is described for zebrafish T-ALL, but it may be applicable to other malignant and non-malignant zebrafish cell types.

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Pruitt, M. M., Marin, W., Waarts, M. R., de Jong, J. L. O. Isolation of the Side Population in Myc-induced T-cell Acute Lymphoblastic Leukemia in Zebrafish. J. Vis. Exp. (123), e55711, doi:10.3791/55711 (2017).

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