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الكشف والتصور من المجمعات البروتين الناجم عن الأضرار الحمض النووي في الثقافات خلية تعليق باستخدام التقريب ربط القرب
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Chapters
- 00:05Title
- 01:02Treatment of Cells and Induction of DNA Damage
- 03:21Cytocentrifugation, Fixation, and Permeabilization
- 05:35Blocking and Primary Antibody Incubation
- 07:19PLA Probes, Ligation, and Amplification
- 09:04Mounting and Imaging
- 10:34Results: The PLA Assay Detects Direct Protein-protein Interactions in Suspension Cell Cultures
- 12:03Conclusion
