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Fraccionamiento de Membranas Cerebrales: Un<em> Ex Vivo</em> Enfoque para evaluar la localización de proteínas subsinápticas

JoVE Journal
Biochemistry
Author Produced

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JoVE Journal Biochemistry

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

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Fraccionamiento de Membranas Cerebrales: Un<em> Ex Vivo</em> Enfoque para evaluar la localización de proteínas subsinápticas

10,197 Views

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09:49 min

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May 12, 2017

DOI:

10.3791/55661-v

DOI:

10.3791/55661-v

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09:49 min

May 12, 2017

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1 Views

Xavier Morató*^1,2, Marc López-Cano*^1,2, Paula M. Canas, Rodrigo A. Cunha, Francisco Ciruela²

¹Unitat de Farmacologia, Departament Patologia i Terapèutica Experimental, Facultat de Medicina, IDIBELL,Universitat de Barcelona, L’Hospitalet de Llobregat, ²Institut de Neurociències,Universitat de Barcelona, ³Center for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine,University of Coimbra

Tags

Brain Membrane Fractionation Synaptic Compartments Synaptic Transmission Electron Microscopy Hippocampus Homogenization Sucrose Calcium Solution Centrifugation Synaptosome Subsynaptic Compartments Detergent Solubilization PH Immunoblot Analysis

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