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新的分析冷痛觉在<em>果蝇</em>幼虫

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Novel Assay for Cold Nociception in Drosophila Larvae

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新的分析冷痛觉在<em>果蝇</em>幼虫

DOI:

10.3791/55568-v

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06:52 min

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April 03, 2017

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Heather N. Turner^2,5, Christian Landry, Michael J. Galko^2,4

¹Department of Genetics,UT MD Anderson Cancer Center, ²Neuroscience Program,Graduate School of Biomedical Sciences at Houston, ³ProDev Engineering, ⁴Genes and Development Program,Graduate School of Biomedical Sciences at Houston, ⁵Section of Neurobiology,University of Southern California

Chapters

00:05Title
00:56Preparation of Larvae
02:31Cold Probe Assay
04:36Results: Larvae Exhibit Unique Behaviors in Response to a Noxious Cold Stimulus
05:36Conclusion

Summary

Automatic Translation

在这里我们展示了一个新的分析来研究果蝇幼虫冷痛觉。该试验利用能够施加焦点有害低温刺激的定制珀尔帖探头和导致量化冷的特定行为。该技术将允许冷痛觉的进一步细胞和分子解剖。

Tags

Keywords: Drosophila Larvae Cold Nociception Noxious Cold Detection Cold Stimulus Cold-evoked Responses Genetic Contexts Third Instar Larvae Cold Probe Temperature Nociception Larvae Sorting Sample Preparation

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