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L'applicazione di geneticamente codificato fluorescente ossido nitrico (NO •) sonde, i geNOps, in tempo reale Imaging di NO • I segnali in singole cellule
JoVE Journal
Biology
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JoVE Journal Biology
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
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L'applicazione di geneticamente codificato fluorescente ossido nitrico (NO •) sonde, i geNOps, in tempo reale Imaging di NO • I segnali in singole cellule

italiano

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12,533 Views

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08:32 min

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March 16, 2017

DOI:

10.3791/55486-v

DOI:
10.3791/55486-v

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08:32 min
March 16, 2017

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6 Views
, , , , , , , , , , , , ,
1Institute of Molecular Biology and Biochemistry,Medical University of Graz

Summary

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This manuscript presents protocols for the application of novel genetically encoded nitric oxide (NO•) probes (geNOps) to monitor single cell NO• fluctuations in real-time using fluorescence microscopy. The Ca2+-triggered NO• formation on the level of individual endothelial cells was visualized by combining geNOps with a chemical Ca2+ sensor.

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Eroglu, E., Rost, R., Bischof, H., Blass, S., Schreilechner, A., Gottschalk, B., Depaoli, M. R., Klec, C., Charoensin, S., Madreiter-Sokolowski, C. T., Ramadani, J., Waldeck-Weiermair, M., Graier, W. F., Malli, R. Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells. J. Vis. Exp. (121), e55486, doi:10.3791/55486 (2017).

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