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उच्च-रिज़ॉल्यूशन एकल कण विश्लेषण, इलेक्ट्रॉन क्रायो-माइक्रोस्कोपी इमेजिंग स्पैयर का उपयोग करना

JoVE Journal
Biochemistry

This content is Free Access.

JoVE Journal Biochemistry

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

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उच्च-रिज़ॉल्यूशन एकल कण विश्लेषण, इलेक्ट्रॉन क्रायो-माइक्रोस्कोपी इमेजिंग स्पैयर का उपयोग करना

49,728 Views

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13:28 min

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May 16, 2017

DOI:

10.3791/55448-v

DOI:

10.3791/55448-v

•

13:28 min

May 16, 2017

•

3 Views

Toshio Moriya, Michael Saur, Markus Stabrin, Felipe Merino, Horatiu Voicu, Zhong Huang, Pawel A. Penczek, Stefan Raunser, Christos Gatsogiannis

¹Department of Structural Biochemistry,Max Planck Institute of Molecular Physiology, ²Department of Biochemistry and Molecular Biology,The University of Texas Medical School at Houston

Tags

Single Particle Analysis Electron Cryo-microscopy SPHIRE Macromolecular Complexes High-resolution Cryo-EM Particle Box Size CTF Estimation Motion Correction Dose-weighted Dose-unweighted Particle Picking

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