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High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

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13:28 min

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May 16, 2017

DOI:

10.3791/55448-v

DOI:
10.3791/55448-v

•

13:28 min
May 16, 2017

•

12 Views
, , , , , , , ,
1Department of Structural Biochemistry,Max Planck Institute of Molecular Physiology, 2Department of Biochemistry and Molecular Biology,The University of Texas Medical School at Houston

Summary

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This paper presents a protocol for processing cryo-EM images using the software suite SPHIRE. The present protocol can be applied for nearly all single particle EM projects that target near-atomic resolution.

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Cite this Article

Moriya, T., Saur, M., Stabrin, M., Merino, F., Voicu, H., Huang, Z., Penczek, P. A., Raunser, S., Gatsogiannis, C. High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE. J. Vis. Exp. (123), e55448, doi:10.3791/55448 (2017).

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