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Peptide Scanning-bijgestaan ​​Identificatie van een monoklonaal antilichaam-erkende Lineair B-celepitoop
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Peptide Scanning-bijgestaan ​​Identificatie van een monoklonaal antilichaam-erkende Lineair B-celepitoop

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08:09 min

March 24, 2017

DOI:

08:09 min
March 24, 2017

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Summary

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Here, the authors present a simple and efficient protocol to define a linear antigenic epitope using a purified monoclonal antibody and peptide scanning through dot-blot hybridization. The identified epitope can then be used in therapeutic and diagnostic applications.

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