Journal
/
Immunology and Infection
/
Human Cells Lung dendritiques: Répartition spatiale et phénotypique identification dans endobronchique Biopsies par immunohistochimie et cytométrie de flux
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry
Please note that all translations are automatically generated. Click here for the English version.

Human Cells Lung dendritiques: Répartition spatiale et phénotypique identification dans endobronchique Biopsies par immunohistochimie et cytométrie de flux

français

Automatically Generated

9,104 Views

•

11:02 min

•

January 20, 2017

DOI:

10.3791/55222-v

DOI:
10.3791/55222-v

•

11:02 min
January 20, 2017

•

1 Views
, , , , , ,
1Immunology and Allergy Unit, Department of Medicine Solna,Karolinska Institutet, 2Division of Medicine, Department of Public Health and Clinical Medicine,Umeå University, 3Divison of Infectious Diseases, Department of Clinical Microbiology,Umeå University

Summary

Automatically generated

Lung-resident immune cells, including dendritic cells (DCs) in humans, are critical for defense against inhaled pathogens and allergens. However, due to the scarcity of human lung tissue, studies are limited. This work presents protocols to process human mucosal endobronchial biopsies for studying lung DCs using immunohistochemistry and flow cytometry.

Explore More Videos

Lung Dendritic CellsEndobronchial BiopsiesImmunohistochemistryFlow CytometryMucosal ImmunologyCOPDSarcoidosisLung CancerSpatial DistributionPhenotypic Identification

Read Article

Cite this Article

Baharom, F., Rankin, G., Scholz, S., Pourazar, J., Ahlm, C., Blomberg, A., Smed-Sörensen, A. Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry. J. Vis. Exp. (119), e55222, doi:10.3791/55222 (2017).

Download .ris file

Copy

Share Video

.

Copy