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Instrueret differentiering af Primitive og endelige hæmatopoietisk ophav fra humane pluripotente stamceller
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Chapters
- 00:05Title
- 00:43Mesoderm Differentiation of hPSCs
- 05:25Specification of CD34+ Hematopoetic Progenitors
- 06:50Enzymatic Dissociation of EBs and Hematopoetic Progenitor Isolation
- 08:23Endothelial-to-hematopoetic Transition
- 10:18T Cell Assay
- 11:38Results: Successful Differentiation of hPSCs
- 14:13Conclusion
Vi præsenterer her, humane pluripotente stamceller (hPSC) kultur protokoller, bruges til at differentiere hPSCs i CD34+ hæmatopoietisk stamfaderen. Denne metode bruger fase-specifikke manipulation af canonical WNT signalering til at angive celler udelukkende til enten det endelige eller primitive hæmatopoietisk program.
