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Mikro Termoforesis kullanarak ATP bir aptamer Bağlama Sitesi Haritalama
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Biochemistry
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JoVE Journal Biochemistry
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
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Mikro Termoforesis kullanarak ATP bir aptamer Bağlama Sitesi Haritalama

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10,331 Views

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08:09 min

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January 07, 2017

DOI:

10.3791/55070-v

DOI:
10.3791/55070-v

•

08:09 min
January 07, 2017

•

5 Views
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12bind GmbH

Summary

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MicroScale Thermophoresis (MST) is a sensitive technology to characterize aptamer-target interactions. This manuscript describes an MST protocol to characterize aptamer-small molecule interactions.

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AptamerATPMicroscale ThermophoresisBinding Site MappingCy5 Labeled DNA AptamerLigand Serial DilutionFluorescence SignalCapillary ScanMolecular Interactions

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Cite this Article

Entzian, C., Schubert, T. Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis. J. Vis. Exp. (119), e55070, doi:10.3791/55070 (2017).

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