Journal
/
Biology
/
Aislamiento y caracterización de microvesículas de la sangre periférica
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Isolation and Characterization of Microvesicles from Peripheral Blood
Please note that all translations are automatically generated. Click here for the English version.

Aislamiento y caracterización de microvesículas de la sangre periférica

español

Automatically Generated

22,497 Views

•

06:03 min

•

January 06, 2017

DOI:

10.3791/55057-v

DOI:
10.3791/55057-v

•

06:03 min
January 06, 2017

•

9 Views
, , , ,
1Department of Hematology/Medical Oncology,University Medical Center Göttingen

Summary

Automatically generated

Extracellular vesicles present in blood have been suggested as novel biomarkers for various diseases. Here, we present a protocol for the isolation of large plasma membrane-derived microvesicles from peripheral blood samples and their subsequent analysis by conventional flow cytometry and Western Blotting.

Explore More Videos

MicrovesiclesPeripheral BloodPlasmaCentrifugationFlow CytometryCharacterizationProtein ConcentrationProinvasiveMetastasisTumor PatientsEDTAPBSFCSAntibody

Read Article

Cite this Article

Menck, K., Bleckmann, A., Schulz, M., Ries, L., Binder, C. Isolation and Characterization of Microvesicles from Peripheral Blood. J. Vis. Exp. (119), e55057, doi:10.3791/55057 (2017).

Download .ris file

Copy

Share Video

.

Copy