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Lichtwaaier fluorescentiemicroscopie van plantenwortels Kweken op het oppervlak van een gel
JoVE Journal
Biology
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JoVE Journal Biology
Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel
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Lichtwaaier fluorescentiemicroscopie van plantenwortels Kweken op het oppervlak van een gel

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14,297 Views

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06:41 min

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January 18, 2017

DOI:

10.3791/55044-v

DOI:
10.3791/55044-v

•

06:41 min
January 18, 2017

•

6 Views
, ,
1Developmental and Cell Biology of Plants,Institute of Science and Technology Austria, 2Bioimaging Facility,Institute of Science and Technology Austria

Summary

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This protocol shows a plant sample preparation method for light-sheet microscopy. The setup is characterized by mounting the plant vertically on the surface of a gel and letting it grow in controlled bright conditions. This allows long-term observation of plant organ development in standardized conditions.

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Cite this Article

von Wangenheim, D., Hauschild, R., Friml, J. Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel. J. Vis. Exp. (119), e55044, doi:10.3791/55044 (2017).

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