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Imaging Metals in Brain Tissue by Laser Ablation – Inductively Coupled Plasma – Mass Spectrometry (LA-ICP-MS)
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Imaging Metals in Brain Tissue by Laser Ablation – Inductively Coupled Plasma – Mass Spectrometry (LA-ICP-MS)

Imaging Metals in Brain Tissue by Laser Ablation – Inductively Coupled Plasma – Mass Spectrometry (LA-ICP-MS)

English

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21,141 Views

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09:05 min

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January 22, 2017

DOI:

10.3791/55042-v

DOI:
10.3791/55042-v

•

09:05 min
January 22, 2017

•

1 Views
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1Elemental Bio-imaging Facility,University of Technology Sydney, 2Florey Institute of Neuroscience and Mental Health,The University of Melbourne, 3Department of Pathology,The University of Melbourne, 4School of Earth Sciences,The University of Melbourne, 5Research School,Ruhr University, 6Department of Physiology,Monash University, 7ESI Ltd., Bozeman, 8Agilent Technologies, Mulgrave

Summary

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Quantitatively mapping metals in tissue by laser ablation - inductively coupled plasma - mass spectrometry (LA-ICP-MS) is a sensitive analytical technique that can provide new insight into how metals participate in normal function and disease processes. Here, we describe a protocol for quantitatively imaging metals in thin sections of mouse neurological tissue.

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Keyword Extract:Laser AblationInductively Coupled PlasmaMass SpectrometryLA-ICP-MSSpatial DistributionMetalsNeurodegenerative DiseasesICPMSLaser Ablation SoftwareBeam DiameterScan SpeedRadiant FluenceAblation AreaSample Scanning

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Hare, D. J., Kysenius, K., Paul, B., Knauer, B., Hutchinson, R. W., O'Connor, C., Fryer, F., Hennessey, T. P., Bush, A. I., Crouch, P. J., Doble, P. A. Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry (LA-ICP-MS). J. Vis. Exp. (119), e55042, doi:10.3791/55042 (2017).

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