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In vitro differentiatie van humane CD4 + FOXP3 + geïnduceerde regulatoire T-cellen (iTregs) van naïeve CD4 + T cellen met behulp van een TGF-β-bevattende Protocol
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
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In vitro differentiatie van humane CD4 + FOXP3 + geïnduceerde regulatoire T-cellen (iTregs) van naïeve CD4 + T cellen met behulp van een TGF-β-bevattende Protocol

Nederlands

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19,654 Views

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08:20 min

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December 30, 2016

DOI:

10.3791/55015-v

DOI:
10.3791/55015-v

•

08:20 min
December 30, 2016

•

10 Views
, , ,
1Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna,Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory

Summary

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Dit protocol beschrijft de reproduceerbare productie en fenotypering van mens veroorzaakte regulerende T-cellen (iTregs) van naïeve CD4 + T-cellen in vitro. Verschillende protocollen voor FOXP3 inductie mogelijk maken voor de studie van specifieke iTreg fenotypes verkregen met de respectieve protocollen.

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Cite this Article

Schmidt, A., Éliás, S., Joshi, R. N., Tegnér, J. In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol. J. Vis. Exp. (118), e55015, doi:10.3791/55015 (2016).

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