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Un protocollo ottimizzato per elettroforetica Mobility Shift Assay Utilizzando oligonucleotidi infrarosso colorante fluorescente etichettati
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Biochemistry
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JoVE Journal Biochemistry
An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
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Un protocollo ottimizzato per elettroforetica Mobility Shift Assay Utilizzando oligonucleotidi infrarosso colorante fluorescente etichettati

italiano

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15,080 Views

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09:58 min

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November 29, 2016

DOI:

10.3791/54863-v

DOI:
10.3791/54863-v

•

09:58 min
November 29, 2016

•

5 Views
, ,
1Department of Biological Sciences,University of Illinois at Chicago

Summary

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Descriviamo qui un protocollo ottimizzato di saggi fluorescenti elettroforetiche Mobility Shift (Femsa) utilizzando purificati SOX-2 proteine ​​insieme con colorante fluorescente marcato sonde di DNA a infrarossi come un caso di studio per affrontare un'importante questione biologica.

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Hsieh, Y., Alqadah, A., Chuang, C. An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides. J. Vis. Exp. (117), e54863, doi:10.3791/54863 (2016).

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