Konjugative Mating Assays für sequenzspezifische Analyse der Übertragung beteiligten Proteine ​​in bakteriellen Konjugation

Here, we present a protocol to knockout a gene of interest involved in plasmid conjugation and subsequently analyze the impact of its absence using mating assays. The function of the gene is further explored to a specific region of its sequence using deletion or point mutations.