Journal
/
Biochemistry
/
Thermostabilization, expressie, zuivering en kristallisatie van het Human serotonine transporter Gebonden aan<em> S</em> citalopram
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram
Please note that all translations are automatically generated. Click here for the English version.

Thermostabilization, expressie, zuivering en kristallisatie van het Human serotonine transporter Gebonden aan<em> S</em> citalopram

Nederlands

Automatically Generated

14,599 Views

•

12:21 min

•

November 27, 2016

DOI:

10.3791/54792-v

DOI:
10.3791/54792-v

•

12:21 min
November 27, 2016

•

9 Views
, ,
1Vollum Institute,Oregon Health & Science University, 2Graduate Group in Biophysics,University of California, San Francisco, 3Howard Hughes Medical Institute,Oregon Health & Science University

Explore More Videos

Serotonin TransporterS-citalopramThermostabilizationExpressionPurificationCrystallizationMembrane ProteinsDrug TargetsNeurotransmitter TransportersReceptorsChannelsSoluble ProteinsHEK293S GnTIDNA TransfectionCitalopramC12MCHSProtease InhibitorsTritiated CitalopramBSAHis-tag AffinitySPA BeadsSertraline

Read Article

Cite this Article

Coleman, J. A., Green, E. M., Gouaux, E. Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram. J. Vis. Exp. (117), e54792, doi:10.3791/54792 (2016).

Download .ris file

Copy

Share Video

.

Copy