/
/
Rápida aquisição de imagens 3D usando de alta resolução episc Microscopia
A subscription to JoVE is required to view this content. Sign in or start your free trial.
7,496 Views
•
07:27 min
•
November 21, 2016
DOI:
1Department of Pediatric Surgery, The Second Affiliated Hospital & Yuying Children’s Hospital,Wenzhou Medical University, 2Departments of Urology,Boston Children’s Hospital, 3Department of Surgery,Harvard Medical School, 4Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute,Guangdong General Hospital, Guangdong Academy of Medical Sciences
Summary
Automatically generated
We describe a detailed protocol using high-resolution episcopic microscopy to acquire three-dimensional (3D) images of mouse embryos. This improved protocol utilizes a modified tissue preparation method to enhance penetration of the fluorescent dye, thereby permitting morphometric analysis of both small and large-sized specimens.
Read Article
Cite this Article
Zhang, H., Huang, J., Liu, X., Zhu, P., Li, Z., Li, X. Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy. J. Vis. Exp. (117), e54625, doi:10.3791/54625 (2016).
Copy