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Fenotypische karakterisering van macrofagen van Rat Kidney door flowcytometrie
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Immunology and Infection
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JoVE Journal Immunology and Infection
Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry
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Fenotypische karakterisering van macrofagen van Rat Kidney door flowcytometrie

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14,411 Views

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07:14 min

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October 18, 2016

DOI:

10.3791/54599-v

DOI:
10.3791/54599-v

•

07:14 min
October 18, 2016

•

12 Views
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1Renal, Vascular and Diabetes Research Lab, IIS-Fundaciòn Jiménez Dìaz,Autonoma University, 2Department of Physiology, Faculty of Medicine,Complutense University, 3Department of Immunology, Centro Nacional de Microbiologìa,Instituto de Salud Carlos III (ISCIII)

Summary

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Dit manuscript beschrijft een gedetailleerd protocol voor fenotypische en kwantitatieve analyse van de aldaar gevestigde macrofagen van rat nieren door middel van flowcytometrie. De resulterende gekleurde cellen kunnen ook worden gebruikt voor andere toepassingen, zoals celsortering, genexpressie analyse of functionele studies, aldus verkregen in het experimentele modelinformatie verhogen.

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Rubio-Navarro, A., Guerrero-Hue, M., Martín-Fernandez, B., Cortegano, I., Olivares-Alvaro, E., de las Heras, N., Alía, M., de Andrés, B., Gaspar, M. L., Egido, J., Moreno, J. A. Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry. J. Vis. Exp. (116), e54599, doi:10.3791/54599 (2016).

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