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Ein<em> In Vitro</em> Modell der Blut-Hirn-Schranke Mit Impedanzspektroskopie: Ein Fokus auf T-Zell-Endothelzellinteraktion
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Immunology and Infection
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JoVE Journal Immunology and Infection
An In Vitro Model of the Blood-brain Barrier Using Impedance Spectroscopy: A Focus on T Cell-endothelial Cell Interaction
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Ein<em> In Vitro</em> Modell der Blut-Hirn-Schranke Mit Impedanzspektroskopie: Ein Fokus auf T-Zell-Endothelzellinteraktion

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11,001 Views

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10:17 min

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December 08, 2016

DOI:

10.3791/54592-v

DOI:
10.3791/54592-v

•

10:17 min
December 08, 2016

•

28 Views
, , , , ,
1Department of Neurology,University Hospital Münster, 2Institute of Biochemistry,University of Münster

Summary

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Here, we describe an in vitro murine model of the blood-brain barrier that makes use of impedance cell spectroscopy, with a focus on the consequences on endothelial cell integrity and permeability upon interaction with activated T cells.

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Blood-brain BarrierIn Vitro ModelImpedance SpectroscopyT Cell-endothelial Cell InteractionNeuroimmunologyAutoimmune CNS DisordersEAEPrimary Endothelial Cell CultureT Cell ActivationMultiple SclerosisMBMEC

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Kuzmanov, I., Herrmann, A. M., Galla, H., Meuth, S. G., Wiendl, H., Klotz, L. An In Vitro Model of the Blood-brain Barrier Using Impedance Spectroscopy: A Focus on T Cell-endothelial Cell Interaction. J. Vis. Exp. (118), e54592, doi:10.3791/54592 (2016).

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