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CD4 hazırlanması<sup> +</sup> Mikroskopi GD3 ve GD2 Gangliozidleri Membran İfade Analizi T hücreleri
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Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
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CD4 hazırlanması<sup> +</sup> Mikroskopi GD3 ve GD2 Gangliozidleri Membran İfade Analizi T hücreleri

Türkçe

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8,300 Views

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10:00 min

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November 08, 2016

DOI:

10.3791/54569-v

DOI:
10.3791/54569-v

•

10:00 min
November 08, 2016

•

2 Views
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1Instituto de Investigación en Ciencias Básicas y Aplicadas, Centro de Investigación en Dinámica Celular, Laboratorio de Glicobiología Humana y Diagnóstico Molecular,Universidad Autónoma del Estado de Morelos

Summary

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Istirahat bölgesindeki gangliosidler ve aktive edilmiş insan naif CD4 + T hücrelerinin membran ekspresyonunu ve sınırlandırılmasını belirlemek için mikroskopla kullanılmak için bir standart antikor boyama protokolü tarif eder. Ayrıca gerçek zamanlı PCR deneyleri <ek düşük giriş RNA kitleri gerekmez 40.000 hücreleri kullanarak tarif edilir.

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Villanueva-Cabello, T. M., Martinez-Duncker, I. Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy. J. Vis. Exp. (117), e54569, doi:10.3791/54569 (2016).

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