Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Nitzan Samra; Yoav Arava

doi:10.3791/54467

Journal

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Genetics

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RNA מחייב רומן בידוד חלבונים לפי שיטת RAPID

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Genetics

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JoVE Journal Genetics

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

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RNA מחייב רומן בידוד חלבונים לפי שיטת RAPID

8,808 Views

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11:19 min

•

September 30, 2016

DOI:

10.3791/54467-v

DOI:

10.3791/54467-v

•

11:19 min

September 30, 2016

•

4 Views

Nitzan Samra^1,2, Yoav Arava¹

¹Faculty of Biology,Technion – Israel Institute of Technology, ²Department of Biomolecular Sciences,Weizmann Institute of Science

Summary

RNA-protein interactions lie at the heart of many cellular processes. Here, we describe an in vivo method to isolate specific RNA and identify novel proteins that are associated with it. This could shed new light on how RNAs are regulated in the cell.

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RNA-binding Proteins RaPID Methodology RNA Metabolism RNA Regulation MRNA-associated Proteins MS2-tagged MRNA MS2 Binding Protein Streptavidin Binding Protein Cross-linking Rapid Purification RNA Degradation Rapid Lysis Buffer Bead-beating Cell Lysate

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RNA מחייב רומן בידוד חלבונים לפי שיטת RAPID

Automatically Generated

Summary

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