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使用F9稀土LACZ基于Cell的报告基因检测在E8.5胚胎和神经球文化相对维甲酸水平的定量测量
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay
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使用F9稀土LACZ基于Cell的报告基因检测在E8.5胚胎和神经球文化相对维甲酸水平的定量测量

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7,646 Views

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09:49 min

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September 06, 2016

DOI:

10.3791/54443-v

DOI:
10.3791/54443-v

•

09:49 min
September 06, 2016

•

1 Views
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1Center for Advanced Biotechnology and Medicine,Rutgers University, 2Department of Neuroscience and Cell Biology,Rutgers University

Summary

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Methods to accurately measure retinoic acid (RA) levels in small amounts of tissue do not exist. This protocol describes the easy, quantitative measurement of relative RA levels in E8.5 embryos and neurospheres using an RA reporter cell line.

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Ababon, M. R., Li, B. I., Matteson, P. G., Millonig, J. H. Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay. J. Vis. Exp. (115), e54443, doi:10.3791/54443 (2016).

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