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Die Bestimmung Unterdrückung des Immunsystems gegen ZNS-Schutz für pharmakologische Interventionen im Bereich der Autoimmun- Demyelinisierung
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Determining Immune System Suppression versus CNS Protection for Pharmacological Interventions in Autoimmune Demyelination
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Die Bestimmung Unterdrückung des Immunsystems gegen ZNS-Schutz für pharmakologische Interventionen im Bereich der Autoimmun- Demyelinisierung

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11,772 Views

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09:38 min

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September 12, 2016

DOI:

10.3791/54348-v

DOI:
10.3791/54348-v

•

09:38 min
September 12, 2016

•

1 Views
, , , ,
1Physical Medicine and Rehabilitation,University of Alabama at Birmingham, 2Department of Pathology,University of Alabama at Birmingham, 3Department of Neurobiology,University of Alabama at Birmingham, 4Center for Glial Biology and Medicine,University of Alabama at Birmingham

Summary

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This protocol describes how to determine whether pharmacological treatments for experimental autoimmune encephalomyelitis show CNS protection as a consequence of suppressing immune cell infiltration or are neuroprotective during the onslaught of immune cell infiltration.

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Immune System SuppressionCNS ProtectionPharmacological InterventionsAutoimmune DemyelinationExperimental Autoimmune Encephalomyelitis (EAE)Multiple SclerosisSystem Xc-transporter InhibitorCell IsolationDensity GradientMyelinCell CultureRestimulation

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Evonuk, K. S., Moseley, C. E., Doyle, R. E., Weaver, C. T., DeSilva, T. M. Determining Immune System Suppression versus CNS Protection for Pharmacological Interventions in Autoimmune Demyelination. J. Vis. Exp. (115), e54348, doi:10.3791/54348 (2016).

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