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Produção em larga escala de cardiomiócitos a partir de células-tronco pluripotentes humanas utilizando um altamente reprodutível pequeno-Based Molecule Protocolo de Diferenciação
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JoVE Journal Developmental Biology
Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

Produção em larga escala de cardiomiócitos a partir de células-tronco pluripotentes humanas utilizando um altamente reprodutível pequeno-Based Molecule Protocolo de Diferenciação

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10,379 Views

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12:21 min

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July 25, 2016

DOI:

10.3791/54276-v

DOI:
10.3791/54276-v

•

12:21 min
July 25, 2016

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1Department of Stem Cells and Developmental Biology, Cell Science Research Center,Royan Institute for Stem Cell Biology and Technology, ACECR, 2Developmental and Stem Cell Biology Division,Victor Chang Cardiac Research Institute, 3St. Vincent´s Clinical School, Faculty of Medicine,University of New South Wales, 4School of Biotechnology and Biomolecular Sciences,University of New South Wales, 5Department of Developmental Biology,University of Science and Culture, 6Heart Centre for Children,The Children´s Hospital at Westmead, 7Sydney Medical School,University of Sydney, 8Department of Developmental Biology,University of Science and Culture, Tehran, Iran

Summary

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Here, we present a robust, fast and scalable cardiomyocyte differentiation protocol for human pluripotent stem cells (hPSCs). Cardiomyocytes derived using this large-scale method can provide sufficient cell numbers for their effective use in human cardiovascular disease modeling, high-throughput drug screening, and potentially clinical applications.

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Keyword Extraction:CardiomyocytesHuman Pluripotent Stem CellsDifferentiation ProtocolHigh EfficiencyReproducibilityCardiac Disease ModelingHuman Induced Pluripotent Stem CellsCost-effectiveHuman Embryonic Stem CellsSingle Cell PassagingSpheroidsPBSTrypsin EDTA

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Fonoudi, H., Ansari, H., Abbasalizadeh, S., Blue, G. M., Aghdami, N., Winlaw, D. S., Harvey, R. P., Bosman, A., Baharvand, H. Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol. J. Vis. Exp. (113), e54276, doi:10.3791/54276 (2016).

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