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고해상도 시간 경과 이미징 및 생활 인간 제대 정맥 내피 세포의 미세 소관 역학의 자동 분석
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Biology
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JoVE Journal Biology
High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells
Please note that all translations are automatically generated. Click here for the English version.

고해상도 시간 경과 이미징 및 생활 인간 제대 정맥 내피 세포의 미세 소관 역학의 자동 분석

English

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10,724 Views

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10:25 min

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August 13, 2016

DOI:

10.3791/54265-v

DOI:
10.3791/54265-v

•

10:25 min
August 13, 2016

•

5 Views
, , ,
1Department of Biological Sciences,University of the Sciences in Philadelphia

Summary

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Protocols for Human Umbilical Vein Endothelial Cell (HUVEC) culture, transient transfection of fluorescently-labeled markers of microtubule growth, live-cell imaging and automated analysis of interphase microtubule growth dynamics are detailed.

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Braun, A., Caesar, N. M., Dang, K., Myers, K. A. High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells. J. Vis. Exp. (114), e54265, doi:10.3791/54265 (2016).

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