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Immunology and Infection

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Een eenvoudige en efficiënte aanpak Mutant Vaccinia Virus vectoren Construct

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

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Een eenvoudige en efficiënte aanpak Mutant Vaccinia Virus vectoren Construct

10,789 Views

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09:16 min

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October 30, 2016

DOI:

10.3791/54171-v

DOI:

10.3791/54171-v

•

09:16 min

October 30, 2016

•

1 Views

Ming Yuan*¹, Pengju Wang*^2,3, Louisa S. Chard, Nicholas R. Lemoine^2,3, Yaohe Wang^2,3

¹Center for Molecular Oncology, Barts Cancer Institute,Queen Mary University of London, ²Sino-British Research Centre for Molecular Oncology, National Center for International Research in Cell and Gene Therapy,Zhengzhou University, ³School of Basic Medical Sciences, Academy of Medical Sciences,Zhengzhou University

Tags

CRISPR-Cas9 Mutant Vaccinia Virus Viral Vector Gene Therapy Vaccination CV-1 Cells Transfection Shuttle Donor Vector Plaque Purification RFP-positive Plaques Cell Lysis Virus Dilution

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