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条纹分析来研究蛋白质底物使用游离海马神经元的吸引力或令人反感的活动
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Neuroscience
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JoVE Journal Neuroscience
Stripe Assay to Study the Attractive or Repulsive Activity of a Protein Substrate Using Dissociated Hippocampal Neurons
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条纹分析来研究蛋白质底物使用游离海马神经元的吸引力或令人反感的活动

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08:11 min

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June 19, 2016

DOI:

10.3791/54096-v

DOI:
10.3791/54096-v

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08:11 min
June 19, 2016

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4 Views
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1Anatomy and Neuroscience,Hamamatsu University School of Medicine, 2Cell and Neurobiology, Zoological Institute,Karlsruhe Institute of Technology (KIT)

Tags

Stripe AssayAxon GuidanceNeuron MigrationNeuron ResponseProtein SubstrateHippocampal NeuronsGuidance MoleculesRecombinant ProteinsProtein CoatingPBSCell CultureLaminin

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Yamagishi, S., Kesavamoorthy, G., Bastmeyer, M., Sato, K. Stripe Assay to Study the Attractive or Repulsive Activity of a Protein Substrate Using Dissociated Hippocampal Neurons. J. Vis. Exp. (112), e54096, doi:10.3791/54096 (2016).

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