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Stripe Assay to Study the Attractive or Repulsive Activity of a Protein Substrate Using Dissociated Hippocampal Neurons
Bölümler
- 00:05Başlık
- 00:46Preparing the Matrices
- 01:43Stripe Generation and Laminin Coating for Neuron Cultures
- 03:59Culturing Hippocampal Neurons fom E15.5 Mouse Embryos
- 06:36Results: Hippocampal Neuron Growth over FLRT2 Coated Substrate
- 07:36Conclusion
Axon guidance molecules regulate neuronal migration and targeted growth-cone navigation. We present a powerful method, the stripe assay, to assess the ability of guidance molecules to attract or repulse neurons. In this protocol, we demonstrate the stripe assay by showing FLRT2's ability to repel cultured hippocampal neurons.
