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May 26, 2016
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We present a protocol to rapidly form giant polymer vesicles (pGVs). Briefly, polymer solutions are dehydrated on dried agarose films adhered to coverslips. Rehydration of the polymer films results in rapid formation of pGVs. This method greatly advances the preparation of synthetic giant vesicles for direct applications in biomimetic studies.
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Greene, A. C., Sasaki, D. Y., Bachand, G. D. Forming Giant-sized Polymersomes Using Gel-assisted Rehydration. J. Vis. Exp. (111), e54051, doi:10.3791/54051 (2016).
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