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Clearing óptico del sistema nervioso central del ratón Utilizando CLARITY pasiva
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Neuroscience
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JoVE Journal Neuroscience
Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY
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Clearing óptico del sistema nervioso central del ratón Utilizando CLARITY pasiva

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13,237 Views

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10:28 min

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June 30, 2016

DOI:

10.3791/54025-v

DOI:
10.3791/54025-v

•

10:28 min
June 30, 2016

•

4 Views
, , ,
1Department of Neurology, David Geffen School of Medicine,University of California, Los Angeles, 2W.M. Keck Science Department,Claremont McKenna, Pitzer & Scripps Colleges

Summary

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Optical clearing techniques are revolutionizing the way tissues are visualized. In this report we describe modifications of the original Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging-compatible Tissue-hYdrogel (CLARITY) protocol that yields more consistent and less expensive results.

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Cite this Article

Roberts, D. G., Johnsonbaugh, H. B., Spence, R. D., MacKenzie-Graham, A. Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY. J. Vis. Exp. (112), e54025, doi:10.3791/54025 (2016).

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