/
/
Combinando Duplo Fluorescência<em> In Situ</em> A hibridação com imunomarcação para a detecção da expressão de três genes em secções do cérebro do rato
A subscription to JoVE is required to view this content. Sign in or start your free trial.
12,733 Views
•
09:23 min
•
March 26, 2016
DOI:
Explore More Videos
Double Fluorescence In Situ HybridizationImmunolabellingGene ExpressionMouse Brain SectionsCell Type LocalizationRNA ProbesFITCDIGTyramideAlkaline PhosphataseFast RedImmunostainingPlasmid DNARNA Polymerase PromotersCDNARestriction EnzymeLinearizationPurificationPhenol-chloroform ExtractionEthanol Precipitation
Read Article
Cite this Article
Jolly, S., Fudge, A., Pringle, N., Richardson, W. D., Li, H. Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections. J. Vis. Exp. (109), e53976, doi:10.3791/53976 (2016).
Copy