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補因子再生と改善された再利用のためのアルギン酸ビーズでマルチ生体触媒の固定化
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Chemistry
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JoVE Journal Chemistry
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
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補因子再生と改善された再利用のためのアルギン酸ビーズでマルチ生体触媒の固定化

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16,940 Views

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09:27 min

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April 22, 2016

DOI:

10.3791/53944-v

DOI:
10.3791/53944-v

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09:27 min
April 22, 2016

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3 Views
, , ,
1Department of Chemical Engineering,Konkuk University, 2Department of Chemical Engineering,University of Michigan

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ImmobilizationMulti-biocatalystsAlginate BeadsCofactor RegenerationReusabilityWhole Cell Biocatalytic SystemBiocatalystsStabilityCofactor RegeneratingE. ColiSpNOxHjLADNADH OxidaseL-arabinitol DehydrogenaseRecombinantIPTGTris-HCl BufferNAD+L-arabinitolL-xylulose

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Gao, H., Khera, E., Lee, J., Wen, F. Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability. J. Vis. Exp. (110), e53944, doi:10.3791/53944 (2016).

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