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LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
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1Center for Cancer Pharmacology,University of Pennsylvania, 2Center for Excellence in Environmental Toxicology,University of Pennsylvania, 3Penn SRP and Department of Systems Pharmacology and Translational Therapeutics,University of Pennsylvania, 4Division of Traumatology, Department of Surgery, Critical Care and Acute Care Surgery,University of Pennsylvania, 5A.J. Drexel Autism Institute,Drexel University
Chapters
- 00:05Title
- 00:38Preparation of Buffers and 100X Stock Solutions
- 01:35Platelet Isolation and Performing an Experiment
- 02:49Quenching and CoA Extraction
- 04:30Results: Analysis of Human Platelets to Study Mitochondrial Metabolism
- 05:21Conclusion
Here we show isolated human platelets can be used as an accessible ex vivo model to study metabolic adaptations in response to the complex I inhibitor rotenone. This approach employs isotopic tracing and relative quantification by liquid chromatography-mass spectrometry and can be applied to a variety of study designs.
