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Differentiation of Atrial Cardiomyocytes from Pluripotent Stem Cells Using the BMP Antagonist Grem2
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Developmental Biology
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JoVE Journal Developmental Biology
Differentiation of Atrial Cardiomyocytes from Pluripotent Stem Cells Using the BMP Antagonist Grem2

Differentiation of Atrial Cardiomyocytes from Pluripotent Stem Cells Using the BMP Antagonist Grem2

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7,645 Views

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08:52 min

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March 10, 2016

DOI:

10.3791/53919-v

DOI:
10.3791/53919-v

•

08:52 min
March 10, 2016

•

2 Views
,
1Department of Medicine, Division of Cardiovascular Medicine,Vanderbilt University School of Medicine, 2Department of Pharmacology,Vanderbilt University, 3Department of Cell & Developmental Biology,Vanderbilt University School of Medicine

Summary

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Generating cardiomyocytes from pluripotent stem cells in vitro allows access to large amounts of cardiac tissue in vitro for basic science and clinical applications. This protocol uses the atrializing factor Grem2 to both increase the numbers of cardiomyocytes obtained and to generate cardiomyocytes with an atrial phenotype.

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Cite this Article

Bylund, J. B., Hatzopoulos, A. K. Differentiation of Atrial Cardiomyocytes from Pluripotent Stem Cells Using the BMP Antagonist Grem2. J. Vis. Exp. (109), e53919, doi:10.3791/53919 (2016).

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